Ordering the Rhythm Plus Hormone Test
Ordering the Rhythm Saliva Hormone Plus helps track hormone patterns over a full day, giving a clear picture of how estrogen, progesterone, testosterone over a month and how cortisol, DHEA,+ melatonin changes over a day. This test uses multiple saliva samples to show how these hormones shift, which can help explain symptoms like fatigue, PMS, or trouble sleeping. Interestingly, this test can also reveal hidden hormone imbalances that may not show up in a single blood draw, making it useful for people with symptoms that come and go.
When ordering the Rhythm Hormone Plus, you can expect these specific benefits:
- Shows how your hormones change throughout the day and night, not just at one moment.
- Helps find patterns linked to sleep problems, energy dips, or mood swings.
- Uses a simple saliva collection kit you can use at home, making it easy and comfortable.
- Gives detailed results for estrogen, progesterone, testosterone, cortisol, DHEA, and melatonin.
- Supports your doctor in making targeted treatment plans based on your unique hormone patterns.
Who Should Consider Hormone and Cortisol Pattern Testing
People who feel tired in the morning but wired at night, or who notice their mood and energy change during the day, may benefit from this test. For example, someone who wakes up feeling exhausted, struggles with focus at work, and then has trouble falling asleep at night could use this test to find out if hormone shifts are part of the problem.
Ordering this test may also be helpful in these situations:
- People with ongoing digestive issues that seem to get worse at certain times of day.
- Those who have headaches or migraines that follow a daily pattern.
- Anyone with skin rashes that flare up in the evening or after stressful events.
- People who notice joint pain or stiffness that changes with their sleep or stress levels.
- Someone who has tried changing their diet or exercise but still feels tired or moody, which can sometimes be linked to hormone cycles.
Testing for hormone and cortisol patterns can help explain why symptoms like fatigue, mood swings, or sleep problems keep coming back, even after trying other solutions. Delaying this test could mean missing the chance to spot hormone changes early, which might make it harder to find the right treatment plan later.
How to Prepare for Hormone and Melatonin Saliva Testing
Fasting is not required for this test, but you should follow the specific timing and collection instructions included in your kit for the most useful results. Always go by the directions your doctor or healthcare provider gives you to make sure your samples are collected correctly and your results are as helpful as possible.
Labs Included When Ordering Your Rhythm Plus Test Kit
| Test Name | Reference Range | What This Test Measures | Low and High Levels of Hormones |
|---|---|---|---|
| Estradiol, Salivary (11 samples) | 1.0 – 4.0 pg/mL (men) 1.3 – 3.3 pg/mL (women, follicular phase) 2.5 – 7.5 pg/mL (women, luteal phase) |
Estradiol is a main form of estrogen, a hormone that affects mood, energy, and reproductive health. This test tracks how estradiol changes throughout the day and night. | High levels mean estrogen may be too high, which can cause bloating, breast tenderness, or mood changes.
Low levels mean estrogen may be too low, which can lead to hot flashes, night sweats, or low energy. |
| Testosterone, Salivary | 30 – 120 pg/mL (men) 10 – 55 pg/mL (women) |
Testosterone is a hormone that supports muscle strength, mood, and focus. This test shows how testosterone levels change during the day. | High levels mean testosterone may be too high, which can cause acne, hair growth, or irritability.
Low levels mean testosterone may be too low, which can cause tiredness, low mood, or trouble building muscle. |
| Progesterone, Salivary (11 samples) | 30 – 100 pg/mL (follicular phase) 75 – 270 pg/mL (luteal phase) |
Progesterone helps balance estrogen and supports sleep and calm feelings. This test tracks progesterone at different times to spot patterns. | High levels mean progesterone may be too high, which can cause sleepiness or bloating.
Low levels mean progesterone may be too low, which can cause trouble sleeping, anxiety, or irregular cycles. |
| Cortisol, Salivary | Morning: 3.7 – 9.5 ng/mL Noon: 1.2 – 3.0 ng/mL Evening: 0.6 – 1.9 ng/mL Night: 0.4 – 1.0 ng/mL |
Cortisol is a stress hormone that should be highest in the morning and lowest at night. This test shows if your daily pattern is balanced. | High levels mean stress may be too high, which can cause trouble sleeping, anxiety, or weight gain.
Low levels mean cortisol may be too low, which can cause tiredness, low blood pressure, or feeling weak. |
| DHEA, Salivary | 100 – 400 pg/mL (men) 50 – 300 pg/mL (women) |
DHEA is a hormone made by the adrenal glands that helps balance other hormones. This test shows if DHEA is in a healthy range for your age and sex. | High levels mean DHEA may be too high, which can cause oily skin, hair changes, or mood swings.
Low levels mean DHEA may be too low, which can cause tiredness, low mood, or trouble handling stress. |
| Melatonin Profile, Salivary | Night: 10 – 80 pg/mL Day: <10 pg/mL |
Melatonin is a hormone that helps control sleep and wake cycles. This test shows if melatonin rises at night and drops during the day as it should. | High levels mean melatonin may be too high, which can cause sleepiness during the day or trouble waking up.
Low levels mean melatonin may be too low, which can cause trouble falling asleep or staying asleep at night. |
Reference ranges may change slightly as labs update their methods or as new research becomes available.
Rhythm Plus FAQ
Is there Rhythm Plus hormone testing near me?
This is a home saliva test kit, so you can collect your samples wherever you are most comfortable. For people with changing symptoms like fatigue or sleep problems, being able to collect samples at home makes it easier to capture your true hormone patterns without the stress of traveling to a lab.
How do I interpret the test results?
While your doctor should review your results with you, we also offer a one-on-one test results review with our clinical team to help you understand your hormone and cortisol patterns in detail.
What is the cost of the test?
The price you see for this test includes standard shipping to your address and return shipping to the lab, though draw fees may apply. Ordering this test can help you find out if hormone changes are behind symptoms like tiredness or mood swings, so you can start feeling better sooner.
How often should I retest?
It’s best to retest every 3 to 6 months, especially if you are starting a new treatment or making changes to your routine, so you can track how your hormone patterns respond over time.
How accurate is the test?
This test uses advanced enzyme immunoassay (EIA) methods for saliva samples, with a specificity of 98% and sensitivity of 97% for hormone detection. TrueHealthLabs.com partners with CLIA-certified and CAP-certified laboratories to uphold rigorous testing standards for dependable results.
Medical Review Board
Reviewed by Jeff Donohue M.D. from Body Logic and Brady Hurst DC, CCCN. Written by True Health Lab’s team of editorial health contributors.
Disclaimer: This information is for educational purposes only and not intended as medical advice. Consult your healthcare provider for personalized guidance.
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